The Rhyothemis princeps Brain Externalization Project

 Lemon balm ( Melissa officinalis ) was found to improve sleep in a fly model of Alzheimer's disease. Screening of sleep assisting drug candidates with a Drosophila model (2020) https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7390450/   "Overall, female flies were more vulnerable to DD stimulation considering total sleep time, but not in the sleep frequency. Therefore, female flies were chosen for the following drug screening study. ... the administration of melatonin shortened sleep latency but did not increase the total sleep time during sleep deprivation ( Fig 4A, 4B and 4F ), indicating that melatonin may only induce flies to sleep faster but not longer. Such results are consistent with human clinical trials that have reported that the prescription of melatonin helps induce sleep but does not enhance total sleep time [ 4 ]. Moreover, the activity index shows no change after melatonin treatment ( Fig 4G ), suggesting that melatonin has a limited effect on activity during wake

Aconitase (aconitate hydratase) is a tricarboxylic acid cycle (TCA) enzyme that converts citrate to iso-citrate via cis-aconitate. It has an iron sulfur cluster that interacts directly with substrate and is prone to oxidation by superoxide. Inactivation of aconitase has been  implicated in neurodegenerative diseases [1]. A mutation in ACO2 is associated with Infantile Cerebellar-Retinal Degeneration [2]. from [2] : "The active (4Fe-4S) cluster was shown to be extremely sensitive to superoxide-mediated inactivation 10 and a decrease in AH activity was observed in several neurodegenerative diseases associated with the development of oxidative stress, in particular Friedreich ataxia [MIM 229300 ], Parkinson [MIM 168600 ], and Alzheimer disease [MIM 104300 ], 11 as well as in mice lacking mitochondrial superoxide dismutase. 12 The reduced AH activity in endomyocardial biopsies of individuals with Friedreich ataxia was attributed not only to oxidative stress but also to the im